Composite
pT7_SigTFp

Part:BBa_K1217004:Design

Designed by: Ma Tsz Shan   Group: iGEM13_Hong_Kong_HKU   (2013-09-15)


Signal sequence fused ppk1 (T.forsythis) under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 421
    Illegal BglII site found at 1883
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1341
    Illegal NgoMIV site found at 1653
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 441


Design Notes

Source

Tannerella forsythia (ATCC 43037)

References